The below is an extract from http://www.irlp.net as a guide to making a IRLP call.
MAKING A DIRECT CONNECTION
First of all listen on your local machine for at least 15 -30 seconds before transmitting and then ask if the repeater is currently in use. Assuming all is clear, identify your self and give the node name or number you wish to call . Example: "VE3xyz for the Sydney node" - - then enter the ON code for the node and release your PTT. Your local repeater should come up with a carrier as it waits for the connection to be authenticated. This can take a few seconds of dead-air so don't be concerned. When the connection is confirmed, the voice ID of the destination node will be transmitted back to you as well as your nodes voice ID to the other repeater.
NOTE: If your node is already connected to another node or reflector, a greeting will play saying; - "your node is currently connected to...ID of the connection") In this case confirm if anyone desires the connection to remain up before dropping by using the OFF code DTMF 73..
Once connected and after hearing the confirming voice ID, wait at least 15 seconds before transmitting as.......
The repeater may be in use, and your entry may have occurred between transmissions.
The voice ID of your node is longer than the voice ID of their node, and the connection is not made until the ID is fully played.
Their computer may be slower, and hence take longer to process the connection than yours.
Press and hold the microphone PTT for a second and then announce your presence and your intention such as you are calling someone specifically or just looking for a QSO with another ham in that city.
If no response is heard, announce your call and your intent to drop the link and then touch- tone in the OFF code DTMF 73. Not a good idea to transmit touch-tone commands without first giving your call-sign. Not only is this courteous it is a regulatory issue in some countries who may be connected to the reflector.
Some nodes are configured so you cannot connect to them if that repeater is active. In this case you will receive the message "The node you are calling is being used locally" If you receive this message wait 5 or 10 minutes and then try again.
If you stay connected to a node and there is no activity on your repeater for 20 minutes, the connection will time out and automatically disconnect with a voice ID disconnect message on both nodes.
The above can also to used when making a Echolink call from your radio. The only difference is adding # in front of the node number you are calling and use #73 to end the call.